Abstract
A new perfusion technique for isolated vascular preparations is described. The cannula inserting method developed by Hongo and Chiba (J. Pharmacol. Methods 9, 83, 1983) and modified by Tsuji and Chiba (Japan. J. Pharmacol. 34, 95, 1984) is useful for observing vascular reactivity in perfused, isolated vessels. In this method, 1) applied substances act only from the intraluminal side; 2) vascular responses to substances, not only constriction but also dilatation in non-treated preparations, are readily obtainable by a single injection of active substances; and 3) the vascular reactivity is reproducible over 7-8 hr at 37 °C. Our modified cannula inserting methods are slso described.
