The effect of NC-1900: Its ameliorating effects on impairments of learning and memory in the CA1 neurons and its intracellular signal transduction pathways

Abstract

Effects of arginine vasopressin (AVP(1-9)) and its analogues on the glycine (Gly)-induced Cl^- currents (I_Gly) were examined in acutely dissociated rat hippocampal CA1 neurons using nystatin perforated and conventional whole-cell patch recording modes under voltage-clamp conditions. The results suggest that the activation of V₁ receptor induces IP₃ production which release Ca²⁺ from IP₃-sensitive Ca²⁺ storage sites. The Ca²⁺ binds to CaM, resulting in theactivation of Ca²⁺/CaM-sensitive adenylate cyclases. Consequently, the activation of PKA inhibits I_Gly. The inhibitory actions of NC-1900 and its analogues on I_Gly might be involved in their ameliorating effects on impairments of learning and memory in. the CA1 neurons.