Abstract
The value of potency of digitalis in frog method changed in accordance with temperature of the test chamber and health condition of frog, notably in winter, it was however, consistent practically when the healthy frogs were kept in thermoconstant chamber at 22±1°C within one or more days, not more than five days, prior to the onset of the assay. In the chamber at 22±1°C, the level of lactate in the blood of frogs which was exposed to winter air, increased remarkably within one or two days, indicating the optimal condition for the assay. The order of the potencies of cardiac glycosides and digitalis leaves which estimated in frog-method was similar to that which determined by cat or pigeon-method, except few principles, e .g. digitoxigenin and digicorin which affected cardiac actions very promptly. The order of the potencies of these drugs in pigeon method was the same as that in cat method. The standard error of the assay in the pigeon was less than that of assay in the cat.
