Abstract
Effect of various drugs containing the anti-inflammatory agents administerd subcutaneously and orally on the increasing activity of connective tissue permeability in rat skin of pronase P, α-chymotrypsin, hyaluronidase and histamine was tested by means of spreading method.
(1) The relative order of the permeability increasing activity of 4 substances showed pronase P>α-chymotrypsin>hyaluronidase>histamine.
(2) Na-salicylate, Aminopyrine, Bucolome, Indomethacin, Flufenamic acid and Prednisolone phosphate showed strong inhibition to the permeability indreasing effect of pronase P. However, Dexamethasone, Chloroquine diphosphate, Diphenhydramine Hcl, Homochlorcyclizine and Cyproheptadine Hcl had a slight or no effect.
(3) The permeability increasing effect of α-chymotrypsin was inhibited strongly by Na-salicylate, Aminopyrine, Bucolome, Indomethacine, and Homochlorcyclizine and Cyproheptadine Hcl which had only slight inhibitory effect against pronase P. On the other hand, Phenylbutazone, Dexamethasone, Chloroquine diphosphate, Diphenhydramine Hcl and ε-aminocaproic acid had a slight or no effect.
(4) Aminopyrine, Bucolome, Indomethacin and Flufenamic acid resulted in strong inhibition on the permeability increasing effect of hyaluronidase.
(5) The increased permeability induced by histamine was antagonized by a number of drugs. Of these drugs, especially Homochlorcyclizine and Cyproheptadine Hcl inhibited strongly the effect of histamine. Na-salicylate, Aminopyrine, Phenylbutazone, Bucolome, Flufenamic acid and Chloroquine diphosphate and Diphenhydramine Hcl which had a slight or no effect to the other substances also inhibited strongly, whereas Indomethacin showed a weak activity only in this case.
