Abstract
Rate of contamination of mitochondria or microsome in plasma membrane fraction was studied by using marker enzymes such as cytochrome c oxidase, succinatecytochrome c reductase, glucose-6-phosphatase, etc..
Results are as follows.
Succinate-cytochrome c reductase and cytochrome c oxidase activities in plasma membrane fraction from normal or CCl4-treated rat liver were less than 4% of those in mitochondria. In membrane, there was no significant increase in glucose-6-phosphatase and RNA located in microsome.
From these results, it appears that increase of NADH-cytochrome c reductase activity in membrane following CCl4 administration dose not result from contamination of subcellular particles during isolation of membrane.
