Abstract
The method, similar to the sucrose gap method in the way of separation, was devised in order to selectively expose drugs to the vas deferens or to the ganglion of the hypogastric nerve-vas deferens preparation of the guinea pig. Two chambers respectively containing the vas and the plexus were separated by the chamber (sucrose chamber) perfused with isotonic sucrose solution. It was assumed that any leakage to the sucrose chamber would result in decrease of the electric resistance of the output-sucrose solution, as the resistance of the input-sucrose solution was far higher than that of Tyrode solution. Therefore, the segregation was checked successively during the experiment by determining a change of electric resistance on the output-sucrose solution. The histological preparation were respectively prepared from the nerve portion from the chamber containing the vas and from the other portion, in order that the existence of the ganglion cell could be observed under light microscopy. The histological observation confirmed the division between the vas and the plexus, and the motor respose of the vas to electrical preganglionic nerve stimulation was inhibited by typical ganglion blockers applied to the plexus, however, such an effect was absent when the same doses of the drugs were applied to the vas. The results suggest that this method would enable selective application of drugs to the vas or to the ganglion.
