Abstract
Leucocytic pyrogen (LP) which is recognized as a mediator of fever reaction induced with bacterial pyrogen could be obtained from the supernatant of the incubation of polymorphonuclear leucocytes (PMNL). On the other hand, many biological active substances could be also obtained from PMNL and such may play an important role in the inflammatory reaction as well as fever reaction. Vascular permeability increasing factor is one of the active substances from PMNL. We have studied the release of Permeability factor (PF) from the incubation of PMNL and some experimental conditions. The following results were obtained; 1) Both PF and LP could be obtained from the crude LP, supernatant of the incubation of PMNL in vitro. 2) PF-3 which expressed as the activity of PF could be detected from 104 cells/ml of PMNL incubation, and FI-3 expressed as the activity of LP could be detected from the incubation of 106 cells/ml of PMNL. 3) FI-3 was more easily inactivated by heating at 56°C-30 min than PF-3. 4) By column chromatography (Biogel P-150) of crude LP, two protein peaks could be detected; in FI-3, the former was stronger than the latter, but in PF-3, the reverse applied. 5) Specific activities of both FI-3 and PF-3 which were divided by protein concentration of their fractions were located in 6.7 × 104 and 1.25 × 104 of the molecular weight of the chromatography as referenced by bovine albumin (M. W. 6.7 × 104) or horse heart cytochrome C (M. W. 1.25 × 104). 6) Activity of PF was not inhibited by pretreatment of cyproheptidine, both an antihistamic and antiserotonic drug.
