Abstract
The effects of anti-ulcer drugs and PGE2 on the proliferation of rat gastric cultured cells were investigated. To study the cell proliferation, the rate of incorporation of 3H-thymidine into the cultured cells was measured. ULTROSER® G, corresponding to fetal bovine serum, was found to dose-dependently increase the incorporation of 3H-thymidine into the cultured cells at a dose range of 1 ?? 4%. The incorporation of 3H-thymidine was highest at 36 hr after the inoculation of cells. Therefore, the effect of the drugs on the proliferation was performed under the condition of 2% ULTROSER®G and evaluated at 36 hr. Aldioxa, cetraxate HCl, cimetidine (10-7 ?? 10-5M) and PGE2 (10-8 ?? 10-6M) had no effect on the cell proliferation, while indomethacin (0.35 ?? 1.41 × 10-3 M) inhibited the proliferation in a dose-dependent manner. Aldioxa, cetraxate HCl (10-5M) and PGE2 (10-6, 10-8M) antagonized the inhibitory effect by indomethacin at a concentration of 0.5 × 10-3M, but cimetidine (10-7 ?? 10-5M) did not. From the above results, it was concluded that this method was useful for investigating the effect of drugs on the proliferation of gastric cultured cells.
