Abstract
Rat hippocampal neurons were cultured on a dish with 64 micro planer electrodes. A complex
network of neurons was formed and the network was able to distinguish patterns of action potentials evoked
by different electrical current inputs. We integrated a living neuronal network and a Khepera II robot or
robot made by LEGO mindstorm NX kit as a body for contacting to outside world. Using self-tuning fuzzy
reasoning, we associated a distinct spatial pattern of evoked action potentials with a particular phenomenon
in the outside of the culture dish. We succeeded in performing collision avoidance behaviour with premised
control rule sets. During collision avoidance, the responding pattern of evoked action potentials was stable
and robust against perturbation to spontaneous network activity. These results suggest that a cultured
neuronal network can represent particular states as symbols corresponding to outside world.
