Abstract
The dissecting microscopic pattern of capillaries and gland openings in cancer and sarcoma of the stomach was studied by a new method utilizing India ink infusion in addition to ordinary observation. Material and Method A total of 162 cases inclusive of 127 with adenocarcinoma, 30 with carcinoma simplex, and 5 with sarcoma were studied: India ink was infused in 8, 1 and 1 respectively. 1) Immediatly after resection the blood vessels were infused with gelatin-added India ink after washing by heparin-added saline solution. 2) The resected stomach was soaked in cold 10% formaimn solution for two hours, and then frozen sections were made in horizontal and perpendicular planes. 3) The forzen section were observed by dissecting microscopy after spraying with methylen blue solution. Result 1) Depressed type of Adenocarcinoma: Gland openings were highly irregular in form and distribution, a capillary running between tow glanc openings. Total capillary picture showed irregular reticulate pattern with vessels of various caliber. 2) Protruded type of Adenocarcinoma: Surface structure showed congregated. pattern of small petal-like projections each of which contained a short arc-shaped capillary. Total capillary picture showed irregular wave-like pattern. 3) Carcinoma simplex: Carcinoma simplex showed hard, rough, and structureless surface. Capillaries are intensely irregular in caliber, form, and distribution, running singly or branchingly on occasion. 4) Reticulumcell: Sarcoma showed similar picture to that of carcinoma simplex.
