Abstract
Cresyl Violet Staining is proved to be useful for magnifying observation of mucosal carcinomas in fresh resected stomachs and furthermore for magnifying endoscopy. For the clinical use of Cresyl Violet Staining, maximal harmless dose of this solution should be clarified. For testing the toxicity of cresyl violet acetate and tnionine solution(0.14% cresyi violet acetate and 0.11% thionine 5% ethanol solution), acute toxic animal test was attempted using wistar strain rats (male ; 45, female ; 20) through two different routes, oral administration and intraperitoneal injection. Rats of these 2 groups were given 100, 25, and 5 times of a clinical dose (5 ml of this solution) by body weight. Blood chemistry (GPT, GOT, LDH, ALP and TB) and histological examinations of liver, kidney and stomach after 24 hours of administration showed no abnormal values or findings. From these results, we concluded that a clinical application of Cresyl Violet Staining was possible.
