Abstract
In order to evaluate the usefulness of Laser Induced Fluorescence Microscopy (LIFM), the microfluorometry of gastric cancer were studied. The fluorescence obtained by LIFMwas compared with that obtained by the standard fluorescence microscopy, using histologicalsections stained with hematoxylin-eosin (HE) and those sections without staining. In sections without staining, only the elastic lamina located in the wall of the vesselshowed strong fluorescence which could not be evaluated by transmission microscopy. In sections stained by HE, the fluorescence were observed in the foveolar cell, themuscularis mucosa, the fibrous connective tissue and the cytoplasm, which were stained byeosin. In particular, the subcellular structure in the cytoplasm of signet ring cell wasclearly revealed. On the other hand, poor fluorescence was observed in the nucleus stainedby hematoxylin. The spectroscopic study of fluorescence revealed that the fluorescence of sectionsstained by HE was those of eosin except the elastic lamina. These observations obtainedby LIFM were also observed by the standard fluorescence microscopy. By these studies, LIFM seems to have few advantage over the standard fluorescence microscopy. LIFMshould be rather applied for microirradiation, more selective microfluorometry and thetime resolved fluorometry than for the standard observation of fluorescence.
