1983 Volume 25 Issue 8 Pages 1178-1187
An endoscope was inserted into the stomach after degultition and peristalsis of the esophagus was inhibited through general paralysis and local paralysis of the pharynx using diazepam and an intravenous injection of a parasympatholytic agent. First, the gastric juice remaining in the stomach from the time of fasting were suctioned out and discarded. The tip of the endoscope was then advanced into the duodenum, and air was then introduced into the duodenum to prevent regurgitation ofduodenal fluid into the stomach. This was followed by washing of the gastric mucosa with water through a tube within a forceps channel with subsequential sampling of the gastric mucus. The gastric mucus was divided into those that pH was adjusted, and those that protein was deproteinized and each type was concentrated. The former was submitted to immunoelectrophoresis and the latter to mucosubstance electrophoresis. Electrophoresis of mucosubstance was conducted following deproteinization by Sevag method in the 1/10 N HCI on cellulose acetate by Wessler method.
