Abstract
Purpose : Clinical usefulness of the peritoneoscopic observation of liver colored with indocyanine green (ICG) has been reported. However, why difference in distribution or local coloration occurs is not understood at all. The present study aims to clarify this point in relation to ligandin. Method : Distribution of ICG of liver surface observed by magnified peritoneoscope (30 min. after injection of 3mg/kg ICG) was compared with the distribution of ligandin in the direct-vision biopsy specimen stained by indirect peroxidase method using anti-ligandin antibody in 34 cases with chronic liver disease. Hepatic GST activities were also measured. Results: 1) Hepatic GST activity which reflects the amount of ligandin decreased with histological progress in chronic liver diseases. 2) Magnified peritoneoscopic observation of liver colored with ICG correlated very well to the lobular structure and also to the distribution of ligandin in the biopsy specimen. Particularly, the patchy markings, the peritoneoscopic finding of early regenerative nodule, were intensively colored with ICG, and ligandin was strongly stained in the aimed biopsy specimen from those patchy markings. Conclusions : These findings suggest that local ICG coloration reflects the change in the distribution or local amount of ligandin derived from the histological changes of liver.
