Abstract
We evaluated the detection rate of Campylobacter pylori in culture, urease test (Christensen'urea medium EIKEN Co Ltd) and acridine-orange stain. In this study, 255 biopsy specimens were taken from 120 patients. Trypticase soy agar added 5% of sheep blood (BBL No 21239) and Chocolate agar (BBL No 21169) were used for culture. In 28% of specimens, C. pylori was detected in all three methods such as culture, urease test and acridine-orange stain. And in 73% of specimens, C. pylori was detected in either culture, urease test or acridine-orange stain. Detection rate of C. pylori was 38% in Chocolate agar, 52% in Trypticase soy agar added 5% of sheep blood, 55% in urease test and 73% in acridine-orange stain. In summary, 1. Acridine-orange stain was the most useful and sensitive method to detect C. pylori among three methods. 2. Culture, urease test and acridine-orange stain should be used together to avoid false negative cases. 3. It is very important to pay special consideration about the detection techniques of C, pylori in order to know "true prevalence rate of C. pylori" and "true relationship between C. pylori and gastroduodenal desease".
