Abstract
The aim of the present study was to investigate the inactivating effect of acidelectrized water(AEW)and peracetic acid(PAA)on the human immunodeficiency virus(HIV). The methods were as follows.1)The inactivation test used HIV solutions that wereprepared by adding HIV to AEW or PAA. Saline was used as the control.2)For thecytotoxicity test, a culture medium was added to AEW or saline instead of HIV. SUPT-1cells was inoculated with the test or control HIV solution and incubated after theinoculum was adjusted to contain 5 x 104 cells/ml. The inactivating effects were studiedusing WST-1 assay as an indicator of intracellular dehydrogenase activity and indirectfluorescent antibody staining to examine HIV antigen expression in the host cell. Cytotox-icity was investigated by comparing dehydrogenase activity in the AEW-or PAA-treatedcells with that in the control cells. Whereas host cells inoculated with saline-treated HIVshowed proliferation on day 4 and reduction in enzyme activity due to HIV proliferationon day 7, HIV, proliferation was observed on both days 4 and 7 for the host cells inoculatedwith AEW-treated HIV. For the host cells inoculated with PAA-treated HIV, enzymeactivity was very low on day 4 and cell proliferation was observed on day 7. No virusantigen-positive cells were detected on indirect fluorescent antibody staining of the cellsinoculated with AEW-or PAA-treated HIV. The cytotoxicity test showed satisfactoryproliferation of the host cells in contact with saline or AEW, but no proliferation of thecells in contact with PAA was evident by day 4, with proliferating cells first appearing onday 7. The results indicate that AEW has the capacity to rapidly inactivate HIV and thatPAA also has an inhibitory effect on HIV, although it also exhibits a cytotoxicic effect.Both AEW and PAA produced an HIV inactivating effect in 30 seconds. The resultspresented would justify the conclusion that AEW and PAA are useful for cleaning anddisinfecting endoscopes and other medical instruments.
