The application of molecular diagnosis for surgical infection

Abstract

In microbiological testing, morphological tests such as smear specimens and biochemical identification after isolation and culture have been the mainstay. Recently, however, mass spectrometry and genetic testing are expected to become new testing instruments. Genetic testing is highly versatile and can detect drug resistance genes and pathogenic genes in addition to detecting microorganisms. The use of genetic testing has been limited due to the need for special techniques and equipment, but with the development of fully automated equipment, its use is expected to increase. It is also useful in the diagnosis of bloodstream infections and postoperative pneumonia, which are important surgical infections. Since genetic testing does not completely replace smear and culture tests, it must be used separately from conventional testing methods. We would like to understand the advantages and disadvantages of genetic testing so that it can be used in the treatment of surgical infections.