1977 Volume 14 Issue 1 Pages 44-49
It has been accepted that amino acids in brain play an important role in metabolism and maintenance of nerve function in brain and vary in the condition of ischemia or hypoxia of the brain. This study measured the contents of amino acids such as glutamate, glutamine, GABA, aspartate, glycine, alanine and taurine in brain during the process of absorption of cerebral hematoma in rats, for the purpose of investigation of the brain metabolism in the repairing process of cerebrovascular accident.
The cerebral hematoma was prepared by local injection of 0.25ml of autologous blood into the region about capsula interna, putamen and caudata nucleus of light cerebral hemisphere of the rat. The amino acids contents were examined with certain interval until 28th day after the preparation of experimental hematoma.
The extent of hematoma in the hemisphere had been maintained equal volume to the injected amount until 3rd day after the preparation and then reduced graduallyto half at the 7th day after. Two weeks after the preparation the hematoma had been almost completely absorbed.
Glutamate content in hematomal (H) and non-hematomal (N-H) hemisphere increased by 33% and 31% on the 3rd day, and 41% and 42% on the 4th day, respectively, and then recovered to normal level. Glutamine content in H and N-H hemisphere markedly increased by 125% and 112% respectively, on the 4th day, and abruptly decreased but maintained high level till third week. GABA content in H and N-H hemisphere also increased by 67% and 100% on the 4th day, and then was normalized. The contents of the other amino acids increased temporarily on the 4th day same as GABA, and recovered to normal level. There was no significant difference in the contents of amino acids between H and N-H hemisphere. No change of amino acids contents in the liver was observed throughout the period of the present study.
The significant increase in amino acids contents at the early stage of absorption of cerebral hematoma may suggest that the amino acids metabolism play an important role in the repairing process of cerebrovascular accident.
