1980 Volume 17 Issue 6 Pages 624-629
Several reports showed that HDL-cholesterol concentration was significantly reduced in patients with cerebral infarction as well as in those with myocardial infarction. A question arises as to whether or not an intervention correcting the abnormally reduced HDL-cholesterol concentration can fortunately protect against these diseases.
To assess the effect of pantethine-a precursor substance of coenzyme A- on lipoprotein abnormalities, it was orally administered to 12 male survivors of cerebral infarction for 3 months in a dose of 1000mg a day. At the beginning of this study, at least 2 months had elapsed after the onset of cerebral infarction and a preceding “wash out” period of one month had elapsed too. Before and after the administration of pantethine, venous blood was drawn and lipoproteins-very low density (VLDL), low density (LDL) and two subfractions of high density (HDL) lipoproteins-were sequentially separated by an ultracentrifugal method according to Havel. Cholesterol was determined by an enzymatic method and triglyceride by an acetylacetone method. Phospholipid was measured by digestion with sulfuric acid and manganese dioxide and protein by Lowry's method.
Serum cholesterol, triglyceride, phospholipid, VLDL and LDL concentrations tended to decrease. The content of triglyceride in LDL was slightly decreased, suggesting the reduction of triglyceride-rich LDL molecule. On the other hand, HDL-cholesterol concentration and HDL:LDL-cholesterol ratio were significantly increased. When HDL was divided into two subfractions, HDL2 and HDL3, HDL2-cholesterol concentration significantly increased, whereas HDL3-cholesterol concentration remained virtually unchanged. Hence, HDL2:HDL3-cholesterol ratio increased to a level of statistical significance. The chemical composition of HDL2 changed slightly: the contents of cholesterol and triglyceride tended to decrease, while the content of phospholipid tended to increase. These results suggest that pantethine gave rise to a significant change in the amount, chemical composition and function of HDL, favoring the antiatherogenic action.