Abstract
1) 14C-cholesterol incorporation into arterial tissues and the release from the tissues were studied under various conditions of the tissue culture. The arterial tissues of various portions of human arteries as well as monkey aorta were used for the experiment. In addition, 14C-cholesterol uptake into the cells was examined at cellular level using the monolayer cultures of chick aortic cells and HeLa cell lines.
2) 14C-cholesterol uptake in human aortic tissue was associated with the incubation time for 36 hours. Moreover, the linear relationship between incorporation rate and incubation time also observed in chick aortic cells for 7 hours and also in HeLa cells for 36 hours.
3) Thus, the incorporation of 14C-cholesterol was shown over 72 hours but the rate decreased gradually afterwards. Contrariwise, the release of 14C-cholesterol from arterial tissue into media was clearly observed for several hours, and then the releasing rate was decreased.
4) The uptake of 14C-cholesterol was compartively higher in the ascending and arch portions than thoracic aorta and the greatest uptake was found in the coronary artery. The incorporation rate in the thoracic aorta trended to be much higher in young subjects than aged ones and it was demonstrably high in monkey thoracic aorta prepared immediately at postmortem.
