Abstract
The effects of acid mucopolysaccharides (1, chondroitin sulfates; 2. sulfated chondroitin sulfate; 3. chondroitin sulfate B and heparan sulfate) on thrombus formation by using a Chandler's technique in connection with the thrombogenic theory of arteriosclerosis were studied. The results using these compounds were as follows.
1) A single intravenous injection of chondroitin sulfates A and C (CS-A and CS-C, 120mg/kg of body weight, each) into 11 and 12 rabbits respectively, prolonged thrombus formation time (TFT), increased the concentrations of hexosamine and galactosamine in plasma, and reduced plasma triglycerides at 60min, after the injection.
When CS-A and CS-C were added to human citrated platelet rich plasma in vitro, TFT was more prolonged with CS-C than that with CS-A.
When CS-C of low and high molecular weights was added to platelet rich plasma in vitro, CS-C of high molecular weight showed prolonged TFT than that of low molecular weight.
2) A single intravenous injection of chondroitin polysulfate (CPS), 30mg/kg of body weight (sulfur: 15.6%), in 18 rabbits resulted in significant differences (P<0.01) between the values of the pre- and 30 and 60min. after the injection, namely prolongation of TFT and reduction in thrombus weight and plasma tri glycerides. These effects were observed somewhat less but still significantly at a dose of 10mg/kg of body weight of CPS, whereas a dose of 5mg/kg did not inhibit thrombus formation.
When chondroitin polysulfates, 15.6% and 9.2% of sulfur-content, were added to plasma in vitro, TFT was strikingly prolonged by CPS (S: 15.6%) than by CPS (S: 9.2%).
A prolonged TFT and a reduced thrombus weight were observed by peroral administrations of CPS (S: 14.1%) 400mg/kg of body weight to rabbits.
3) When chondroitin sulfate B and heparan sulfate were added to plasma in vitro, prolongation of the TFT was proved.
4) In the clinical study, TFT showed a slight acceleration in the plasma of the patients with cerebral thrombosis than in the subjects with myocardial infarction or cerebral haemorrhage. But the difference proved not significant.
