The Japanese Journal of Genetics
Online ISSN : 1880-5787
Print ISSN : 0021-504X
ISSN-L : 0021-504X
INDUCTION OF MULTINUCLEATE CELLS IN GRASSHOPPER SPERMATOCYTES BY TREATMENT WITH AURAMIN
STUDIES ON ABNORMAL NUCLEAR DIVISION 7
KEN-YA KAWAMURA
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1953 Volume 28 Issue 1 Pages 8-11

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Abstract
Auramin which was dissolved in Ringer-Locke-Barta's solution at concentration of 0.01%, was injected in the abdomen of the grasshopper (Podisma sapporense), 0.02cc in each. After 30 minutes, the course of division was followed in living spermatocytes by the hanging-drop method, with the aid of a phase-contrast microscope.
By application of auramin the cells tend to fuse with one another into giant cells and this leads to the formation of the multinucleate cell. In this present study, the process of the formation of the multinucleate cells was successively traced in a single cell.
Evidence was presented to show that the mitochondria plays an important role in the division of the cell body.
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© The Genetics Society of Japan
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