Abstract
The relation between genome constitution and flavonoid ingredients was investigated in Triticum and its related genera with the aid of two-dimensional paper chromatography. In all six genera were examined, namely Triticum (representatives of all 3 groups), Aegilops, Agropyrum, Haynaldia, rye and barley. The methanol extracts of fresh leaves were chromatographed. The solvents used were (1) organic phase of o-cresol-iso-propanol-water (5:1: 4) and (2) 10% acetic acid. Lead subacetate was found to be most effective as color reagent. All chromatograms were composed of yellowish and brownish spots; also a few spots of fluorescent substances were observed. The number of spots per chromatogram ranged from 7 to 13. Although identification of pigment spots was not carrid out, a certain relation was found between the pigment system and genome constitution of the examined materials. Within the genus Triticum, the pigment spots produced by one species seemed for the most part to correspond to those found in others, and to increase in number with the increasing number of genomes. Furthermore, spots produced by Agropyrum and Aegilops were partially similar to those of Triticum. Haynaldia and rye showed almost identical patterns of pigment spots, in particular with regard, to the two major pigments. However, they differed markedly from those of Triticum. Barley seemed to be quite different from all other examined genera.
