Abstract
Twelve amylase negative mutants were isolated from Bacillus subtilis 168 and its derivatives by irradiating spores with ultraviolet light. Using these mutants as recipients, genetic analyses of gene controlling the production of amylase in B. subtilis were performed by DNA transformation. Agar gel electrophoretic study showed that genealogically different strains carried different amylase genes and resulted in the production of amylases with different electrophoretic mobility. Data obtained indicate that these amylase genes are allelic to each other and a site named E within the structure gene specifies the molecular forms of each amylase protein.
