Abstract
Twenty eight species including woody and succulent plants were tested for the isolation of intact protoplasts. From leaves intact cells or protoplasts were isolated by using Macerozyme followed by Cellulase, which was suitable for most of dicotyledons, or by using a mixture of Macerozyme and Cellulase, which was suitable for monocotyledons and for some dicotyledons. Many intact protoplasts were isolated from calli by using a mixture of Colonase and Cellulase. About half of protoplasts isolated from rice callus were multinucleate. From the petals, many colored cells or protoplasts were isolated.
The effect of Zymolyase on the isolation of protoplasts from pollen tetrads was examined. This enzyme was very effective for isolation of protoplasts from pollen tetrads or from pollen mother cells, but not effective for digesting mature pollen grains.
