INDUCED DIFFERENTIATION OF BUCKWHEAT PLANTS FROM SUBCULTURED CALLUSES IN VITRO
1974 Volume 49 Issue 3 Pages 139-146
Details
1974 Volume 49 Issue 3 Pages 139-146
For callus induction and restoration of plants from callus tissue of buckwheat, kinetin showed no effect, but supplements of 2, 4-D (10mg/l) to White's basal medium induced calluses from germinating seeds. Callus tissues subcultured for 48 months grew vigorously on the modified White's basal medium.
Nearly 40.5% of cells of callus tissues which were subcultured 6-36 months had the diploid chromosome number 16. The cell population became much more uniform in chromosome number after a longer period of subculture.
Shoot and root formation were not found on the proliferating calluses in White's basal medium and modified white's basal medium. However, transfer to modified RM-1964 medium after one or two months resulted in a tendency towards organ-forming capacity.
The five restored plants obtained showed normal chromosome constitution.
