IDENTIFICATION OF MONOSOMIC AND NULLISOMIC OATS USING LEAF PEROXIDASE ISOZYMES

Abstract

In order to identify specific monosomics and nullisomics, twenty one monosomics and four nullisomics of hexaploid oats were investigated for their peroxidase isozymes in flag leaves by gel electrofocusing method. Five monosomics (i. e., Mk 3, 4, 9, 22 and 24) and four nullisomics (i. e., N9, 11, 24 and 26) of Kanota oats could be distinguished from the disomic line by peroxidase isozymes. A larger number of the bands decreased their activities in all the nullisomics examined. Although the peroxidase isozymic systems of hexaploid oats are thought to be very complex, analysis of peroxidase isozymes is useful for identification of some monosomics and nullisomics in hexaploid oats.