Abstract
Variation of chromosome number and constitution in the callus cells of Luzula elegans Lowe (syn. L. purpurea Link.) (2n=6) was investigated. The chromosomes of the plant have nonlocalized kinetochores. Callus was induced and subcultured on the basal medium of Linsmaier and Skoog (1965) with 3.0mg/l of 2, 4-D and 300mg/l of casein hydrolysate, and with 3.0mg/l of 2, 4-D and 1g/l of yeast extract. The ratio of increase in fresh callus weight was about three and a half times after two months of subculture on the medium with casein hydrolysate. The ratio of increase on the medium with yeast extract was larger than that on the medium with casein hydrolysate. Aneuploid cells and new type chromosomes were observed. Most new type chromosomes were medium-sized fragment and dot chromosome.
