Abstract
Chinese hamster V79 cells were treated with cyclophosphamide (CP) and various concentrations of S-15 mix in vitro, and then examined for chromosome aberrations, sister chromatid exchange (SCE) and induction of 8-azaguanine (8AG)-resistant mutations. Cells from hamster embryos treated with CP in utero by intraperitoneal injection were tested for drug-resistant mutation. CP caused a marked dose-dependent increase of chromosome aberrations, SCE and drug resistant mutations in V79 cells in the presence of lower concentrations of S-15. The inductions of 8AG-resistant mutations in V79 cells by CP with 1% S-15 were 7.4 times higher than those in the control. Trans- placentral treatment with CP also caused a marked increase in 8AG-resistant mutations of hamster embryonic cells.
