Abstract
A new acetate-requiring mutant was isolated in Neurospora crassa and termed as ace-8. The ace-8 gene is located on the left arm of the linkage group VII near the centromere between thi-3 and qa-3 genes. The ace-8 mutant strain lacks the activity of pyruvate kinase (EC 2.7.1.40). Accumulation of glycolytic intermediates is consistent with the enzyme lesion. The mutant strain is extremely sensitive to glycolytic sugars, glucose and sucrose, but grows well on a medium containing gluconeogenic carbon sources, acetate, ethanol and alanine.
