Coat color genes of D-amino acid oxidase deficient ddY/DAO^- mice and nonlinkage of gene for D-amino acid oxidase to coat color genes

Abstract

Coat color genes of mutant ddY/DAO^- mice that do not have D-amino acid oxidase were examined by crosses of these mice with NC (AAbbCC) and II TES (aabbCCddss) mice. All the (ddY/DAO^-×NC)F₁ mice had agouti coats and the (ddY/DAO^-×II TES)F₁ mice had black coats. These results indicate that the ddY/DAO^- mice carry aaBBcc DDSS gene.
Linkage relationship of Dao-1 gene coding for D-amino acid oxidase to coat color genes was examined. Segregants produced from backcrosses of (ddY/DAO^-×IITES)F₁ with ddY/DAO^- and (ddY/DAO^-×NC)F₁ with ddY/DAO^- were examined for D-amino acid oxidase activity and coat color. F₂ segregants from a (ddY/DAO^-×NC) cross were also studied. These crosses showed that Dao-1 was not linked to a, b or c.