Abstract
Single cells in a free and suspended state were induced from the tissue cultured shoot primordia of Haplopappus gracilis (2n=4). New shoot primordia were formed from the suspensions of single cells, when the suspensions were cultured in the conditions of original shoot primordia reported by Tanaka and Ikeda (1983). A new path of micropropagation in somatic life cycle in plant tissue culture was proposed, that is shoot primordia→suspensions of single cells→regenerated shoot primordia→plantlets→shoot primordia.
