Analysis of transcripts of aminoglycoside phosphotransferase II and nopaline synthase in Nicotiana glauca transformants mediated by recombinant Ti plasmid

Abstract

The vector possessing two marker genes encoding for, aminoglycoside phosphotransferase II (APH(3′)II) and nopaline synthase (NOS) was inserted into the octopine-type Ti plasmid through homologous recombination. Following the confirmation of foreign gene expression in Nicotiana glauca tumor cells with respect to kanamycin resistance and nopaline synthesis, the analysis was made on the transcripts. There was a quantitative difference in the amount of mRNA between APH(3′)II and NOS. The size of mRNA for APH(3′)II and NOS were 1250 bases and 1550 bases, respectively.