Abstract
Thermophilic bacteria are one of the most attractive forms of life, and their adaptation mechanisms to elevated temperatures have been extensively studied over the years. Thermal adaptations cell components such as proteins and RNA are well studied, but adaptations of interactions between these components must be also vital for the thermophiles. Protein-DNA interactions play crucial roles in the cell, but little is known about their thermal adaptations. In this study, we analyzed DNA-binding proteins from thermophilic bacteria. Comparison of amino acid compositions the DNA-binding interfaces between thermophiles and their rnesophilic close relatives revealed several commonalities between phylogenetically unrelated organisms. Advantages and limitations our methods will be also discussed.
