Abstract
We measured ADA activity of lymphocytes in the joint fluid and the peripheral blood for studying whether the immune system is correlated to the purine metabolic system. Method: T-and B-cells in lymphocyte were separated from the peripheral blood and the joint of patients with RA. Enzyme assay is following. The sample is sonicated for 25 sec. at 20KHz and is added to reaction mixture (0.2 mM Tris-HC1 buffer (pH 7.4),3.0 mM [8-14C]adenosine and the enzyme sample). Incubati on times were 30 min. at 37°C. After incubation the reaction mixture is applied over DEAE-cellulose paper. Radioactivity of the sample is measured with liquid scintilation counter. Result: 1. ADA activity of T cells (n mol/hr 106 cells) in the joint fluid is higher than that of B cells. (T: B = 1.6: 1) 2. ADA activity of T cells in peripheral blood is higher than that of B cells. (T: B=1.4: 1) 3. ADA activity of T and B cells in the joint fluid is 1.69 times as high as that in the peripheral blood. And we could get some ideas that the immune system would be related to the purine metabolic system.
