Abstract
We have purified adenylate cyclase (AC) and cytidylate cyclase (CC) from rat brain. The nucleotide cyclase activities were evaluated by determining cyclic AMP or cyclic CMP by enzyme immunoassay (EIA) which had been developed in our laboratory.
Triton X-100 (0.3%) soluble fractions containing AC and CC were subjected to dodecyl - Sepharose 4 B column chromatography. Further purification was carried out with Sepharose 6 B gel filtration. AC and CC were separated by affinity chromatography with ATP- and CTP- Sepharoses. These findings suggested that AC and CC are different enzyme species.
