Abstract
we report high-performance liquid chromatographic method with column switching for urinary orotidine. Analysis was carried out with a reversed-phase column and an anion-exchange column connected by a column switch and controlled automatically by a computerized system controller. Each sample required only 20 min and 100 samples could be analyzed consecutively. The relationship between standard concentrations and peak heights was linear in the concentration range from 1-1000μmol/l. The recovery of orotidine added to urine was between 96.8% and 100.8%. With our method urinary orotidine can be measured simply and accurately.
