Abstract
The timothy leaf spot disease caused by Cladosporium phlei (Gregory) de Vries (Syn. Heterosporium phlei Gregory) has been reported to distribute in a wide area and to bring not a little damage to timothy grown in Hokkaido. When timothy is infected with the fungus, small violet spots on its leaf surface are observed as a symptom. Though the pigment produced by the fungi belonging to Dematiaceae have been described, little is known about the pigment produced by C. phlei and their toxicity for grasses and domestic animals. As a fundamental study, this paper deals with the purification procedure of an orange pigment from C. phlei and its some chemical characteristics.