Abstract
Sucrose and phlein hydrolases were demonstrated to exist in the haplocorm of timothy. The hydrolyzing activities were examined using the soluble and insoluble fractions. The soluble fraction was cell sap freed from mitochondria, and the insoluble fraction was fibrous materials including cell walls. The activities were mainly found in the insoluble fraction. However, it remains to be investigated whether the hydrolases are adsorbed to the insoluble fraction during the preparation of haplocorm homogenate. Neither the soluble nor the insoluble fraction was active to inulin.