Abstract
The sporulation process was observed in timothy eye spot fungus, Heterosporium phlei GREGORY. 0.5ml of spore suspension was poured on to the surface of V8-juice agar in a petri dish with a sterilized Komagome pipet. Sporulation was observed to initiate 36 hours after the pipetting (Plate II). Sporulation increased rapidly between 48 and 60 hours (Fig.1). Morphologically uniform spores were available 5-6 days after the pipetting (Fig.2). The suspention was consisted of 45-55% of one septate spores, 15-20% of spores with no septa, about 20% of two septate spores, and about 10% of three septate spores (Table 1 and Fig.2). Spores were the larger in size and extruded the more germtubes as the number of septa increased. While the number of germtubes per cell decreased as the number of septa increased. Attached conidia germinated easily in damp air (Plate 1). The formation of the first and the second conidia was observed microscopically (Plate 3).
