Abstract
In this study, the changes in peroxidases from timothy leaves by the infection of Cladosporium phlei were examined. Four peroxidase fractions (P-1a, P-1b, P-2 and P-3) were separated using SP-Sephadex C-50 and DEAE-Sephadex A-50 column chromatography. Optimal pHs of P-1a, P-1b, P-2 and P-3 were 5.5, 6.0, 5.5 and 5.0, respectively, regardless of the kind of leaves used. P-1a, P-1b and P-3 were increased markedly by the infection of this fungus, but P-2 was not. Ten and 12 bands having peroxidase activities were detected in healthy and infected leaves, respectively, by disc electrophoresis. Among the various compounds tested, some phenolics and aromatic amines were oxidized in the presence of H_2O_2 solution by a main peroxidase fraction (P-1a).
