Abstract
The changes in sucrose and fructosan hydrolyzing activities (SHA and FHA) associated with the insoluble fraction in the haplocorm of timothy were examined during the regrowth after cutting. SHA increased during the first 2 days, followed by decrease till 4th day and thereafter kept almost constant, while FHA was not altered by cutting. Some properties of these enzymes were also tested. The optimal pHs for SHA and FHA were 4.5 and 6.0, respectively, the pH activity curve of FHA being broad within the range of pH 5.0-7.0. SHA was most active at around 30℃. β-Fructofuranosidase, α-glucosidase, β-glucosidase and cellulase were bound to the insoluble fraction. The release of SHA from the insoluble fraction was partially attained by treatment with papain, but not by 1M NaCl, 0.25M borate buffer, cellulase, bromelin and pronase. SHA remained in the insoluble fraction was increased only by treatment with cellulase.
