Abstract
Effect of abscisic acid (ABA) on the induction of sucrose and fructosan hydrolyzing activities (SHA and FHA) in the haplocorm of timothy (Phleum pratense L.) after cutting was examined. ABA (10μl of 0.75mM per plant) was applied on the cutting surface of the stem. Total SHA increased in the non-treated haplocorm, but not in the treated one. Total FHA appeared first in both of the non-treated and treated haplocorms after 10 days, the increasing rate being almost the same between them. SHA was separated into 5 fractions (A, B, C, D and E) by DEAE Sephadex A-50 column chromatography. SHA increased in all fractions excluding E in the non-treated haplocorm at 4th day, while only in A and C fractions in the treated one. The differences were also observed between the protein concentrations in the non-treated and treated haplocorms in each fraction. The development of new shoots from the haplocorm was retarded by ABA treatment.
