Abstract
Simple determination method of feed starch was investigated by using two enzymes, such as pronase and amylase (α-amylase). Seven corn silages, seven formula feeds (for dairy cattle, swine and poultry), corn grain, wheat grain, barley grain, soybean meal, corn gluten feed, peanut meal and soy sauce cake were used as feed samples. At first, organic cellular contents (OCC) was determined by successive treatments of amylase and pronase. Next, in another experiment, pronase-soluble organic matter (POM) was determined. OCC contains crude protein, crude fat, sugars, organic acids, starch and other solubles. On the other hand, POM contains crude protein, crude fat, sugars, organic acids and other solubles. For this reason, the value of OCC minus POM is equal theoretically to strach content of feed sample. Actually, highly significant correlations were obtained between the value of OCC minus POM and starch contents in three feed groups (tables 2 and 3). Starch content of feed can be predicted simply and exactly by these enzymatic system and the regression equation in tables 2 and 3.
