Abstract
For establishing tissue culture methods of Rhodesgrass (Chloris gay ana Kunth. cv. Katambora), callus induction and plant regeneration from mesocotyl-explants were investigated. The most effective medium for callus induction was MURASHIGE and SKOOG'S medium modified with 1.0 mg/l 2,4-dichloro-phenoxyacetic acid (2,4-D), 200 mg/l myo-inositol and 1.0 mg/l thiaminef HCl. The mesocotyl-explants for callus induction were incubated in a growth chamber controlled at 25℃ under the darkness. A high frequency of plantlet formation was obtained from the yellowish compact-friable calli with the nodular structures. The most effective medium for plant regeneration was MURASHIGE and SKOOG'S hormone-free medium. The effects of added activated charcoal and yeast extract for plant regeneration were not recognized. The calli for plant regeneration were incubated in a growth chamber controlled at 25℃ under the about 3000 lux light intensity and 12-hour day length.
