Abstract
It has been reported that alfalfa deproteinised juice (DPJ) has a comparatively small amount of sugars, and also it has some soluble substances that prevent yeast from growing luxuriously. But in the present experiment, almost the same amount of yeast yield was obtained on DPJ by steam coagulation when compared to the yield from mixed juice of malt, yeast extracts, glucose and peptone. Yeast yields were less on DPJ which was obtained from green juice (GJ) fermented anaerobically than from that of steam coagulation ; addition of glucose or (NH_4)_2SO_4 and KH_2PO_4 to it was not effective. But, when it was treated with resin Diaion HP-20 yeast yields were improved, and supplementation of (NH_4)_2SO_4 and KH_2PO_4 after treatment with Diaion resulted much more yeast yields. The DPJ obtained from glucose added and anaerobically fermented GJ did not prevent yeast growth and was better than that of fractionated DPJ by steam coagulation as the yeast culture. The best yeast yield was obtained when it was treated with Diaion HP-20 and then added (NH_4)_2SO_4 and KH_2PO_4. Although treatment with Diaion HP-20 improved a little yeast yields, but it is not suitable practically from economical point of view for unconcentrated DPJ in green crop fractionation (GCF) process.
