Abstract
Extraction and detection methods of 4-methylimidazole (4-MI) of ammoniated-roughage have been studied. Recovery of 4-MI from ammoniated-roughage was higher in ion-pair extraction method compared with sodium carbonate-chloroform-ethanol extraction method and hydrochloric acid-chloroform-ethanol extraction method. there was no difference among the ion-pair high performance liquid chromatography (HPLC) method, dansylated sample-HPLC method and acetylated sample-gaschromatography-mass spectrometry (GC-MS) method on 4-MI values determined.
