Abstract
We examined tue usefulness of isozumes for the clone identification of Trifolium repens to enable to determine the extent and pattern of its clonal spread in fields. Twenty-one enzyme systems were examined using starch gel electrophoresis for the nine buffer systems (ranging from pH 5.5-8.6) to select appropriate ones. Of these enzymes, three ones (aconitase (ACO), esterase (EST), and phosphoglucoisomerase (PGI)) showed high resolution and were highly polymorphic. The examination of the extent of intra-cultivar (cv. Grasslands Huia) variation in these enzymes strongly indicated the usefulness of the isoaymes for the clone identification. Using this method, we analysed the clone structure of a T. repens mini-sward population, distinguishing well genets intermingled each other. A practical procedure for the clone identification in fields will be also proposed.
