Abstract
Avicelase (F1-3), xylanase (F1-5), pectinase (F5-1) and carboxymethylcellulase (CMCase) (F5-2) of Acremonium cellulolyticus Y-94(ACS2) were fractionated by anion-ex-changechromatography (Q Sepharose High Performance: Pharmacia) and hydrophobic chromatography (Phenyl Sepharose High Performance: Pharmacia) and investigated for their effect on fermentation quality of ensiling alfalfa. Each of the enzyme preparations simply showed a specific cell-wall degrading activity. Fresh alfalfa was cut and chopped to a 2 cm length and ensiled on treatments with avicelase and commercial lactate producing bacteria, alone or combined with each of the other enzyme preparations, for 14 days at 26℃ in nylon/polyethlene bags. Alfalfa ensiled with the F1-3 fraction produced more lactic acid than did the untreated one. Enhanced production of lactic acid in the F1-3 treated silages was observed for combined treatments with the F5-1 or F5-2 fractions, while the combination with the F1-5 fraction was less effective. Treatments with cellulases and pectinase used in this study can increase lactic acid production on ensiled alfalfa.
