Abstract
The generation of haploids in most grass species is relatively infrequent, but has continually been considered a potential tool for the development of fixed inbred lines, a method for cytoplasmic transfer, or polyploid genome manipulations. This study was originally undertaken to identify the frequency of successful hybridization following crosses between Poa arachnifera×P secunda. However, during the research, four androgenic haploids representing the polyploid P.secunda parent were obtained with varying chromosome numbers. Each of the haploids exhibited the fibrous root system characteristic of the P secunda parent. The generation of androgenic haploids offers an opportunity to observe P.secunda germplasm in a P arachnifera cytoplasm as well as an opportunity to utilize such materials in geneticbstudies of polyploid P.secunda.